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Sunday, April 19, 2020 | History

3 edition of Single molecule dynamics in life science found in the catalog.

Single molecule dynamics in life science

Single molecule dynamics in life science

  • 315 Want to read
  • 15 Currently reading

Published by Wiley-VCH in Weinheim .
Written in English

    Subjects:
  • Biology -- Laboratory manuals,
  • Molecules,
  • Molecular Biology -- methods,
  • Models, Molecular,
  • Molecular Structure

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by Toshio Yanagida and Yoshiharu Ishii.
    GenreLaboratory manuals
    ContributionsYanagida, Toshio., Ishii, Yoshiharu.
    Classifications
    LC ClassificationsQH317 .S46 2009
    The Physical Object
    Paginationxviii, 328 p. :
    Number of Pages328
    ID Numbers
    Open LibraryOL23632494M
    ISBN 103527312889
    ISBN 109783527312887
    LC Control Number2009275882

    Elf, J., Li, G. W. & Xie, X. S. Probing transcription factor dynamics at the single-molecule level in a living cell. Science , – (). This article probes gene-regulation dynamics by following the search time of transcription factors to their targets on DNA in living cells. Analytes include single small molecules, single biopolymers—such as DNA and RNA, single peptides and proteins, single viruses and bacteria, and single nanoparticles. The key issue in the development of practical sensors based on single-molecule sensing methods is the manner in which transportation of single molecules into sensors may be achieved. Mar 24 NMR studies of dynamics: spin relaxation, chemical exchange and H/D J3 Mar 31 IR and Raman spectroscopy E Apr 7 Apr 14 Molecular dynamics simulations. Theory and practice of force-field based studies of macromolecules I Apr 21 Apr 28 Optimal microscopy: light, fluorescence and atomic force microscopy, single molecule studies F May 12? Toinvestigate the picosecond dynamics ofa single molecule, wecoupled a MHz train of ultrashort light pulses (5-ps FWHM, nm, bandwidth.


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Single molecule dynamics in life science Download PDF EPUB FB2

Get this from a library. Single molecule dynamics in life science. [Toshio Yanagida; Yoshiharu Ishii;] -- Yanagida (Osaka University Graduate School of Frontier Biosciences) and Ishii (Core Research for Evolutional Science and Technology, Japan Science and Technology Agency) gather pioneers in the field.

In this first comprehensive resource on the topic, one of the world's most distinguished single molecule scientists has put together a team of pioneers in the field to show how to both set up and interpret a single molecule experiment.

Following an introduction to single molecule measurements and enzymology, the authors consider molecular Author: Toshio Yanagida.

Get this from a library. Single molecule dynamics in life science. [Toshio Yanagida; Yoshiharu Ishii;] -- In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single.

Force-clamp Spectroscopy of Single Proteins. Prof. Dr Verlag GmbH & Co. KGaA. Book Title. Single Molecule Dynamics in Life Science. Additional Information. How to Cite. Dougan, L., Brujic, J. and Fernandez, J. () Force-clamp Spectroscopy of Single Proteins, in Single Molecule Dynamics in Life Science (eds T.

Yanagida and Y. Ishii. Book Editor(s): Prof. Toshio Yanagida. Osaka University, Graduate School of Frontier Bioscience and Graduate School of Medicine, 1‐3 Yamada‐oka, Suita, Osaka ‐, Japan Single‐molecule FRET. smFRET Studies of Rep Helicase. SmFRET Studies of Holliday Junction. Outlook.

References. Single Molecule Dynamics in Life Science Author: Taekjip Ha. Dec 04,  · Enzymatic turnovers of single cholesterol oxidase molecules were observed in real time by monitoring the emission from the enzyme's fluorescent active site, flavin adenine dinucleotide (FAD).

Statistical analyses of single-molecule trajectories revealed a significant and slow fluctuation in the rate of cholesterol oxidation by FAD. The static disorder and dynamic disorder of reaction rates.

Mar 15,  · These results suggest that some lamin A dynamics may be linked to disease type, especially with regards to cardiac dysfunction and systemic progeria. However, we do not find a universal correlation between single molecule dynamics and specific types of laminopathies.

Analysis of distinct Halo-lamin A populationsCited by: 1. Mar 26,  · Furthermore, DNA dynamics influence complex, subtle, and time-dependent protein behaviors on short spatial and temporal scales, a swath of which are only accessible through single-molecule methodologies.

(Recent reviews that explore the nature of Cited by: Applications. In life sciences, single-particle tracking is broadly used to quantify the dynamics of molecules/proteins in live cells (of bacteria, yeast, mammalian cells and live Drosophila embryos).

It has been extensively used to study the transcription factor dynamics in live cells. Dec 07,  · This book provides a representative selection of recent developments in the rapidly evolving field of single molecule techniques of importance in life sciences and will have future impact on the quan- tative description of biological processes.

The editors of this book hope that the chapters, written by leading scientists in the field, will. In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set.

Single molecule detection requires a highly sensitive confocal microscope. For a simple set-up, a single laser and a single photon counting detector is needed. For more sophisticated experiments, several detectors as well as a highly stable piezo scanning system are used. Book chapter Full text access Chapter 2 - Alternating laser excitation spectroscopy of freely diffusing single molecule: Applications to bimolecular structure, dynamics and interactions E.

Nir. Dec 12,  · We have recently developed a fluorescence-imaging approach to investigate RNA dynamics in living cells at the single-molecule level.

This approach entails the use of molecular beacons (MBs), which are a class of stem-loop forming oligonculeotide-based probes that emit detectable fluorescence upon binding to target sequence, and tandem repeats Author: Mingming Chen, Shiqi Mao, Xiaotian Wu, Zhao Ma, Yantao Yang, Christopher J.

Krueger, Christopher J. The Gordon Conference on Single-Molecule Approaches to Biology will provide an interdisciplinary forum for researchers who develop single-molecule methodologies and apply them to solve important problems in biology and beyond.

Single-molecule FRET Studies of Helicases and Holliday Junctions. / Ha, Taekjip. Single Molecule Dynamics in Life Science. Wiley-VCH Verlag GmbH & Co.

KGaA, p. Author: Taekjip Ha. The range of techniques and methods has steadily grown since the first report of Single Molecule Detection (SMD) in Today, single molecules can be detected using either wide field or confocal fluorescence microscopy, Scanning Nearfield Optical Microscopy (SNOM), Atomic Force Microscopy (AFM), or Raman scattering.

Feb 26,  · Single-molecule experiments (SMEs) have contributed various tools to the build and design of highly sensitive and precise scientific instruments.

Medical Home Author: News Medical. Sep 04,  · Abstract. Single-molecule methods have emerged as powerful tools in life science research. These techniques allow the detection and manipulation of individual biological molecules and investigate, with unprecedented resolution, their conformations and dynamics at the nanoscale ggl14i.top by: 2.

Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert.

It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties.

Transporting individual molecules across cell membranes is a fundamental process in cellular metabolism. Although the crystal diffraction technique has greatly contributed to our understanding of the structures of the involved transporters, a description of the dynamic transport mechanism at the single-molecule level has been extremely elusive.

A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the ggl14i.top: Yoshiharu Ishii.

UNSW’s Single Molecule Science initiative focuses on transforming medicine by providing a molecular perspective on complex biological systems and processes, encompassing biophysics, biochemistry and cell biology as well as nanotechnology and nanofabrication.

Learn biology dynamics life chapter 9 with free interactive flashcards. Choose from different sets of biology dynamics life chapter 9 flashcards on Quizlet. Abstract: Single-molecule techniques have transformed dramatically the way we think about biophysics, making it possible to address questions about the dynamics of systems in equilibrium that were practically unthinkable just a decade ago.

This review focuses on how single-molecule fluorescence and fluorescence correlation techniques have Cited by: Molecular dynamics (MD) is a computer simulation method for analyzing the physical movements of atoms and ggl14i.top atoms and molecules are allowed to interact for a fixed period of time, giving a view of the dynamic "evolution" of the system.

In the most common version, the trajectories of atoms and molecules are determined by numerically solving Newton's equations of motion for a system.

Single molecule experiments are not only important for biophysics, but also for chemistry, physics, and materials science. The analysis of single molecule data often becomes complicated because of the noise or fluctuations present (both Gaussian and non-Gaussian) and the lack of a theoretical framework for processes far from equilibrium.

He pioneered research on single-molecule spectroscopy and its application to biology. His team was the first to detect FRET between a single donor and a single acceptor molecule, a technique that is now widely used to study the structure and conformational dynamics of macromolecules.

Single molecule biology is an inherently cross-disciplinary field of research, so this book appeals to workers from a broad range of disciplines, including structural biologists, biophysicists, cell biologists, and biochemists.

The target audience is advanced undergraduates, graduates, postgraduates, and. Single-molecule spectroscopy is a powerful approach to analyze protein conformational dynamics under physiological conditions, providing dynamic perspectives on a molecular-level understanding of protein structurefunction ggl14i.top by: 7.

Nov 02,  · From artificial surfaces to living cells, Molecular Nano Dynamics, Vol. I and Vol. II explores more than 40 important methods for dynamic observation of the nanoscale.

Edited by absolute science greats from Japan, this two-volume set covers all important aspects of this topic: nanoscale spectroscopy and characterization tools, nanostructure dynamics, single living cell dynamics, active Author: Hiroshi Fukumura.

This chapter summarizes a series of new single-molecule spectroscopy investigations in the life sciences at Cornell University that began with our invention of Fluorescence Correlation Spectroscopy (FCS) about Our invention of FCS became my first focus on the "Molecular Dynamics of Life." It motivated my transition from research on quantum fluctuations and transport in condensed matter Cited by: 2.

The observation and manipulation of individual molecules is one of the most exciting developments in modern molecular science. Single Molecule Science: Physical Principles and Models provides an introduction to the mathematical tools and physical theories needed to understand, explain, and model single-molecule observations.

This book explains the physical principles underlying the major Manufacturer: CRC Press. Summary. The observation and manipulation of individual molecules is one of the most exciting developments in modern molecular science. Single Molecule Science: Physical Principles and Models provides an introduction to the mathematical tools and physical theories needed to understand, explain, and model single-molecule observations.

This book explains the physical principles underlying the. Jan 22,  · Scientists for the first time have developed a single molecule that can absorb energy from the entire visible spectrum of light, meaning it can harness over 50% more solar energy than current.

In this study, we use the EcoRII restriction enzyme, which employs a three-site binding mechanism to catalyze cleavage of a single recognition site. Using high-speed atomic force microscopy (HS-AFM) to image single-molecule interactions in real time, we were able to observe binding, translocation, and dissociation mechanisms of the EcoRII Cited by: Oct 15,  · Buy Single Molecule Dynamics in Life Science by Toshio Yanagida, Yoshiharu Ishii from Waterstones today.

Click and Collect from your local Waterstones Pages: Life Science Network is an internet platform for professional networking and sharing of information and knowledge in life sciences.

The platform integrates multiple functional modules. One of the modules is dedicated to life science jobs, which features a job board for. Lecture Single- molecule Dynamics: rotation, diffusion, energy transfer, and powerful application in biological systems Why and how to study molecule dynamics: rotation and diffusion.

2 research cases. Single-molecule fluorescence resonance energy transfer (FRET) and application in protein dynamic structural imaging. Imaging of long- range molecule diffusion: monitoring virus.

The aim of this volume is to provide a broad overview of single-molecule approaches applied to biomolecules. Chapters in Single Molecule Techniques: Methods and Protocols, Second Edition detail the most widely used single-molecule techniques, such as DNA, DNA-binding proteins, motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology.

Learn chapter 6 biology dynamics life chemistry with free interactive flashcards. Choose from different sets of chapter 6 biology dynamics life chemistry flashcards on Quizlet.In recent years, the development of single molecule detection (SMD) techniques has opened up a new era of life science.

The dynamic properties of biomolecules and the unique operations of.Mar 13,  · Single-molecule trace analysis confirmed that the average residence time of Sox2 on a DNA probe containing a canonical Sox2 binding site is s while the average residence time on a non Probing transcription factor dynamics at the single-molecule level in a living cell.

Science (New York, NY. ; Science (New York, ggl14i.top by: